Alkaline Phosphatase and Calcification: Histochemical Investigations on the Relationship between Alkaline Phosphatase and Calcification

Pyrophosphate, alkaline phosphatase, and vascular calcification.

Vascular Calcification To the Editor: In their editorial on vascular calcification, Demer and Tintut1 incorrectly state that pyrophosphate reduces calcification by inhibiting alkaline phosphatase. Actually, the opposite is true. Alkaline phosphatase promotes calcification by reducing pyrophosphate levels. Pyrophosphate is a substrate for alkaline phosphatase,2 and this enzyme regulates pyrophos...

Histochemical Localization of Alkaline Phosphatase in The

CHEMICAL investigations have shown that alkaline phosphatase is present in the retina (De Conciliis, 1934; Reis, 1951). In these investigations the whole retina was homogenized and the enzyme estimated in extract. Obviously the estimations of enzyme activity by such a necessarily gross method give no idea of the exact distribution of the enzyme in this, functionally, so finely differentiated ti...

Histochemical and biochemical studies on leukocyte alkaline phosphatase activity.

Relationship between alkaline phosphatase and neomycin formation in Streptomyces fradiae.

Studies on phosphatase activity of Streptomyces fradiae 3535 grown in three different media indicate that neomycin formation varies directly with enzyme activity, sodium nitrate-maltose-mineral salts medium giving the highest yields of alkaline phosphatase and neomycin. S. fradiae contains more than one alkaline phosphatase and the phosphatase responsible for hydrolysis of neomycin phosphate ap...

Fluoride and Alkaline Phosphatase

Since serum alkaline phosphatase increases in fluoride therapy for osteoporosis, it is generally accepted that fluoride stimulates bone formation. However, histochemical studies have shown that alkaline phosphatase is also increased in resorbing osteocytes. Fluoride is toxic to metabolically active bone cells, alkaline phosphatase is released, and serum alkaline phosphatase increases. We propos...